Digestion of Repair Sites in Rat Liver DNA by Endogenous Nucleases

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avondale-bepress-to-dspace.facultyScience
avondale-bepress-to-dspace.peer_review_statusPeer reviewed before publication
avondale-bepress.abstract<p>The proportion of sheared rat liver DNA recovered from benzoylated DEAE-cellulose in the final stage following stepwise elution with NaCl and caffeine solutions was dependent upon the DNA isolation procedure. An increase in the proportion of DNA containing single stranded regions, consequent upon delay or addition of Mg2+ prior to phenol extraction, suggested nuclease mediated degradation. Administration of methyl methanesulphonate to rats resulted in a consistent proportional increase in the caffeine-eluted fraction. The results of caffeine gradient elution of control and alkylated DNA from benzoylated DEAE-cellulose were consistent with repair-associated single stranded regions being substrates for endogenous single strand-specific exonucleases.</p>
avondale-bepress.articleid1055
avondale-bepress.authorsEwan Ward
avondale-bepress.authorsBernard W Stewart
avondale-bepress.authorsMichelle Haber
avondale-bepress.context-key6387409
avondale-bepress.coverpage-urlhttps://research.avondale.edu.au/sci_math_papers/56
avondale-bepress.document-typearticle
avondale-bepress.field.author_faculty_disciplineScience
avondale-bepress.field.comments<p>Used by permission: <a href="http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291521-6551">Wiley</a></p> <p><em>Biochemistry International now IUBMB Life.</em></p> <p>Copyright © 1986 Academic Press Australia<em><br /></em></p> <p>At the time of writing <em>Ewan Ward</em> was affiliated with the <em>Queensland Institute of Medical Research</em>.</p>
avondale-bepress.field.custom_citation<p>Ward, E.J., Stewart, B.W., & Haber, M. (1986). Digestion of repair sites in rat liver DNA by endogenous nucleases. <em>Biochemistry International, 13</em>(5), 903-913.</p>
avondale-bepress.field.embargo_date2014-11-23T00:00:00Z
avondale-bepress.field.field_of_education01 Natural and Physical Sciences
avondale-bepress.field.for111201 Cancer Cell Biology
avondale-bepress.field.issn0158-5231
avondale-bepress.field.issue_number5
avondale-bepress.field.journalBiochemistry International
avondale-bepress.field.page_numbers903-913
avondale-bepress.field.peer_reviewBefore publication
avondale-bepress.field.publication_date1986-11-01T00:00:00Z
avondale-bepress.field.source_publication<p>This article was originally published as:</p> <p>Ward, E.J., Stewart, B.W., & Haber, M. (1986). Digestion of repair sites in rat liver DNA by endogenous nucleases. <em>Biochemistry International, 13</em>(5), 903-913.</p> <p>ISSN: 0158-5231</p>
avondale-bepress.field.staff_classificationPermanent
avondale-bepress.field.volume_number13
avondale-bepress.fulltext-urlhttps://research.avondale.edu.au/cgi/viewcontent.cgi?article=1055&amp;context=sci_math_papers&amp;unstamped=1
avondale-bepress.keywordsmethyl methanesulphonate
avondale-bepress.keywordscaffeine
avondale-bepress.keywordsbenzoylated DEAE-cellulose
avondale-bepress.keywordsendogenous single strand-specific exonucleases
avondale-bepress.label56
avondale-bepress.publication-date1986-11-01T00:00:00Z
avondale-bepress.publication-titleScience and Mathematics Papers and Journal Articles
avondale-bepress.statepublished
avondale-bepress.submission-date2014-11-23T14:41:33Z
avondale-bepress.submission-pathsci_math_papers/56
avondale-bepress.titleDigestion of Repair Sites in Rat Liver DNA by Endogenous Nucleases
avondale-bepress.typearticle
dc.contributor.authorHaber, Michelle
dc.contributor.authorStewart, Bernard W.
dc.contributor.authorWard, Ewan
dc.date.accessioned2023-11-01T00:38:46Z
dc.date.available2023-11-01T00:38:46Z
dc.date.issued1986-11-01
dc.date.submitted2014-11-23T14:41:33Z
dc.description.abstract<p>The proportion of sheared rat liver DNA recovered from benzoylated DEAE-cellulose in the final stage following stepwise elution with NaCl and caffeine solutions was dependent upon the DNA isolation procedure. An increase in the proportion of DNA containing single stranded regions, consequent upon delay or addition of Mg2+ prior to phenol extraction, suggested nuclease mediated degradation. Administration of methyl methanesulphonate to rats resulted in a consistent proportional increase in the caffeine-eluted fraction. The results of caffeine gradient elution of control and alkylated DNA from benzoylated DEAE-cellulose were consistent with repair-associated single stranded regions being substrates for endogenous single strand-specific exonucleases.</p>
dc.description.versionBefore publication
dc.identifier.citation<p>Ward, E.J., Stewart, B.W., & Haber, M. (1986). Digestion of repair sites in rat liver DNA by endogenous nucleases. <em>Biochemistry International, 13</em>(5), 903-913.</p>
dc.identifier.issn0158-5231
dc.identifier.urihttps://research.avondale.edu.au/handle/123456789/06387409
dc.language.isoen_us
dc.provenance<p>This article was originally published as:</p> <p>Ward, E.J., Stewart, B.W., & Haber, M. (1986). Digestion of repair sites in rat liver DNA by endogenous nucleases. <em>Biochemistry International, 13</em>(5), 903-913.</p> <p>ISSN: 0158-5231</p>
dc.rights<p>Used by permission: <a href="http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291521-6551">Wiley</a></p> <p><em>Biochemistry International now IUBMB Life.</em></p> <p>Copyright © 1986 Academic Press Australia<em><br /></em></p> <p>At the time of writing <em>Ewan Ward</em> was affiliated with the <em>Queensland Institute of Medical Research</em>.</p>
dc.subjectmethyl methanesulphonate
dc.subjectcaffeine
dc.subjectbenzoylated DEAE-cellulose
dc.subjectendogenous single strand-specific exonucleases
dc.titleDigestion of Repair Sites in Rat Liver DNA by Endogenous Nucleases
dc.typeJournal Article

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