Grant, RossCroft, Kevin D.Mori, Trevor A.Hokin, BevanBilgin, AyseGuest, Jade2023-11-012023-11-012015-09-142020-12-14<p>Guest, J., Bilgin, A., Hokin, B., Mori, T., Croft, K., & Grant, R. (2015). Novel relationships between B12, Folate and markers of inflammation, oxidative stress and NAD(H) levels in the CNS and plasma of a healthy human cohort. <em>Nutritional Neuroscience,18</em>(8), 355-364. doi: 10.1179/1476830515Y.0000000041</p>1476-8305https://doi.org/10.1179/1476830515Y.0000000041https://research.avondale.edu.au/handle/123456789/20598543<p><strong>Objective</strong>: To evaluate the relationship between folate, cobalamin (Cbl), and homocysteine (Hcy), and markers of inflammation and oxidative stress within the periphery and central nervous system (CNS) of a healthy human cohort.</p> <p><strong>Methods</strong>: Thirty-five matched cerebrospinal fluid (CSF) and plasma samples were collected from consenting participants who required a spinal tap for the administration of anaesthetic. Plasma concentrations of Hcy and both plasma and CSF levels of folate, Cbl, nicotinamide adenine dinucleotide (NAD(H)) and markers of inflammation (interleukin-6, IL-6), and oxidative stress (F₂-isoprostanes, 8-hydroxy-2′-deoxyguanosine (8-OHdG), and total antioxidant capacity (TAC)) were quantified.</p> <p><strong>Results</strong>: In the peripheral circulation, positive associations were observed between plasma folate and Cbl, and plasma TAC (<em>P</em> ≤ 0.01; <em>P</em> ≤ 0.01) and plasma NAD(H) (<em>P</em> ≤ 0.05; <em>P</em> ≤ 0.05) levels, respectively. Plasma folate was inversely associated with plasma Hcy concentrations (<em>P</em> ≤ 0.05); however, no statistically significant relationships were observed between plasma Hcy and plasma markers of inflammation, oxidative stress, or [NAD(H)].</p> <p>Within the CNS plasma Hcy correlated positively with CSF IL-6 (<em>P</em> ≤ 0.01) and negatively with CSF NAD(H) (<em>P</em> ≤ 0.05) concentrations. An inverse association was observed between CSF folate and CSF levels of IL-6 (<em>P</em> ≤ 0.05). Unexpectedly, a positive association between CSF Cbl and CSF 8-OHdG levels was also found (<em>P</em> ≤ 0.01).</p> <p><strong>Discussion</strong>: These results indicate that folate and Cbl concentrations may influence the levels of oxidative damage, inflammation, and NAD(H), both systemically and within the CNS.</p>en-us<p>Due to copyright restrictions this article is unavailable for download.</p> <p>The full text of this article may be accessed via ResearchGate <a href="https://www.researchgate.net/publication/280906558_Novel_relationships_between_B12_folate_and_markers_of_inflammation_oxidative_stress_and_NADH_levels_in_the_CNS_and_plasma_of_a_healthy_human_cohort">here</a></p>CobalaminFolateHomocysteineInflammationOxidative stressNicotinamide adenine dinucleotideCentral nervous systemBrainJournal Article