Novel Relationships between B12, Folate and Markers of Inflammation, Oxidative Stress and NAD(H) Levels, Systemically and in the CNS of a Healthy Human Cohort

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avondale-bepress-to-dspace.facultyNursing
avondale-bepress-to-dspace.peer_review_statusPeer reviewed before publication
avondale-bepress.abstract<p><strong>Objective</strong>: To evaluate the relationship between folate, cobalamin (Cbl), and homocysteine (Hcy), and markers of inflammation and oxidative stress within the periphery and central nervous system (CNS) of a healthy human cohort.</p> <p><strong>Methods</strong>: Thirty-five matched cerebrospinal fluid (CSF) and plasma samples were collected from consenting participants who required a spinal tap for the administration of anaesthetic. Plasma concentrations of Hcy and both plasma and CSF levels of folate, Cbl, nicotinamide adenine dinucleotide (NAD(H)) and markers of inflammation (interleukin-6, IL-6), and oxidative stress (F<sub>2</sub>-isoprostanes, 8-hydroxy-2′-deoxyguanosine (8-OHdG), and total antioxidant capacity (TAC)) were quantified.</p> <p><strong>Results</strong>: In the peripheral circulation, positive associations were observed between plasma folate and Cbl, and plasma TAC (<em>P</em> ≤ 0.01; <em>P</em> ≤ 0.01) and plasma NAD(H) (<em>P</em> ≤ 0.05; <em>P</em> ≤ 0.05) levels, respectively. Plasma folate was inversely associated with plasma Hcy concentrations (<em>P</em> ≤ 0.05); however, no statistically significant relationships were observed between plasma Hcy and plasma markers of inflammation, oxidative stress, or [NAD(H)].</p> <p>Within the CNS plasma Hcy correlated positively with CSF IL-6 (<em>P</em> ≤ 0.01) and negatively with CSF NAD(H) (<em>P</em> ≤ 0.05) concentrations. An inverse association was observed between CSF folate and CSF levels of IL-6 (<em>P</em> ≤ 0.05). Unexpectedly, a positive association between CSF Cbl and CSF 8-OHdG levels was also found (<em>P</em> ≤ 0.01).</p> <p><strong>Discussion</strong>: These results indicate that folate and Cbl concentrations may influence the levels of oxidative damage, inflammation, and NAD(H), both systemically and within the CNS.</p>
avondale-bepress.articleid1216
avondale-bepress.authorsJade Guest
avondale-bepress.authorsAyse Bilgin
avondale-bepress.authorsBevan Hokin
avondale-bepress.authorsTrevor A Mori
avondale-bepress.authorsKevin D Croft
avondale-bepress.authorsRoss Grant
avondale-bepress.context-key20598543
avondale-bepress.coverpage-urlhttps://research.avondale.edu.au/nh_papers/226
avondale-bepress.document-typearticle
avondale-bepress.field.author_faculty_disciplineNursing
avondale-bepress.field.avon_earlyonline2015-08-11T00:00:00-07:00
avondale-bepress.field.comments<p>Due to copyright restrictions this article is unavailable for download.</p> <p>The full text of this article may be accessed via ResearchGate <a href="https://www.researchgate.net/publication/280906558_Novel_relationships_between_B12_folate_and_markers_of_inflammation_oxidative_stress_and_NADH_levels_in_the_CNS_and_plasma_of_a_healthy_human_cohort">here</a></p>
avondale-bepress.field.custom_citation<p>Guest, J., Bilgin, A., Hokin, B., Mori, T., Croft, K., & Grant, R. (2015). Novel relationships between B12, Folate and markers of inflammation, oxidative stress and NAD(H) levels in the CNS and plasma of a healthy human cohort. <em>Nutritional Neuroscience,18</em>(8), 355-364. doi: 10.1179/1476830515Y.0000000041</p>
avondale-bepress.field.doihttps://doi.org/10.1179/1476830515Y.0000000041
avondale-bepress.field.email_boxtrue
avondale-bepress.field.embargo_date2020-12-14T00:00:00Z
avondale-bepress.field.field_of_education06 Health
avondale-bepress.field.for1109 NEUROSCIENCES
avondale-bepress.field.for_2021420899 Traditional, complementary and integrative medicine not elsewhere classified
avondale-bepress.field.issn1476-8305
avondale-bepress.field.issue_number8
avondale-bepress.field.journalNutritional Neuroscience
avondale-bepress.field.page_numbers355-364
avondale-bepress.field.peer_reviewBefore publication
avondale-bepress.field.publication_date2015-09-14T00:00:00Z
avondale-bepress.field.reportable_itemsC1
avondale-bepress.field.research_centreLifestyle and Health Research Centre
avondale-bepress.field.source_fulltext_urlhttps://doi.org/10.1179/1476830515Y.0000000041
avondale-bepress.field.staff_classificationConjoint
avondale-bepress.field.volume_number18
avondale-bepress.keywordsCobalamin
avondale-bepress.keywordsFolate
avondale-bepress.keywordsHomocysteine
avondale-bepress.keywordsInflammation
avondale-bepress.keywordsOxidative stress
avondale-bepress.keywordsNicotinamide adenine dinucleotide
avondale-bepress.keywordsCentral nervous system
avondale-bepress.keywordsBrain
avondale-bepress.label226
avondale-bepress.publication-date2015-09-14T00:00:00Z
avondale-bepress.publication-titleNursing and Health Papers and Journal Articles
avondale-bepress.statepublished
avondale-bepress.submission-date2020-12-14T16:44:04Z
avondale-bepress.submission-pathnh_papers/226
avondale-bepress.titleNovel Relationships between B12, Folate and Markers of Inflammation, Oxidative Stress and NAD(H) Levels, Systemically and in the CNS of a Healthy Human Cohort
avondale-bepress.typearticle
dc.contributor.authorGrant, Ross
dc.contributor.authorCroft, Kevin D.
dc.contributor.authorMori, Trevor A.
dc.contributor.authorHokin, Bevan
dc.contributor.authorBilgin, Ayse
dc.contributor.authorGuest, Jade
dc.date.accessioned2023-11-01T00:32:18Z
dc.date.available2023-11-01T00:32:18Z
dc.date.issued2015-09-14
dc.date.submitted2020-12-14T16:44:04Z
dc.description.abstract<p><strong>Objective</strong>: To evaluate the relationship between folate, cobalamin (Cbl), and homocysteine (Hcy), and markers of inflammation and oxidative stress within the periphery and central nervous system (CNS) of a healthy human cohort.</p> <p><strong>Methods</strong>: Thirty-five matched cerebrospinal fluid (CSF) and plasma samples were collected from consenting participants who required a spinal tap for the administration of anaesthetic. Plasma concentrations of Hcy and both plasma and CSF levels of folate, Cbl, nicotinamide adenine dinucleotide (NAD(H)) and markers of inflammation (interleukin-6, IL-6), and oxidative stress (F<sub>2</sub>-isoprostanes, 8-hydroxy-2′-deoxyguanosine (8-OHdG), and total antioxidant capacity (TAC)) were quantified.</p> <p><strong>Results</strong>: In the peripheral circulation, positive associations were observed between plasma folate and Cbl, and plasma TAC (<em>P</em> ≤ 0.01; <em>P</em> ≤ 0.01) and plasma NAD(H) (<em>P</em> ≤ 0.05; <em>P</em> ≤ 0.05) levels, respectively. Plasma folate was inversely associated with plasma Hcy concentrations (<em>P</em> ≤ 0.05); however, no statistically significant relationships were observed between plasma Hcy and plasma markers of inflammation, oxidative stress, or [NAD(H)].</p> <p>Within the CNS plasma Hcy correlated positively with CSF IL-6 (<em>P</em> ≤ 0.01) and negatively with CSF NAD(H) (<em>P</em> ≤ 0.05) concentrations. An inverse association was observed between CSF folate and CSF levels of IL-6 (<em>P</em> ≤ 0.05). Unexpectedly, a positive association between CSF Cbl and CSF 8-OHdG levels was also found (<em>P</em> ≤ 0.01).</p> <p><strong>Discussion</strong>: These results indicate that folate and Cbl concentrations may influence the levels of oxidative damage, inflammation, and NAD(H), both systemically and within the CNS.</p>
dc.description.versionBefore publication
dc.identifier.citation<p>Guest, J., Bilgin, A., Hokin, B., Mori, T., Croft, K., & Grant, R. (2015). Novel relationships between B12, Folate and markers of inflammation, oxidative stress and NAD(H) levels in the CNS and plasma of a healthy human cohort. <em>Nutritional Neuroscience,18</em>(8), 355-364. doi: 10.1179/1476830515Y.0000000041</p>
dc.identifier.doihttps://doi.org/10.1179/1476830515Y.0000000041
dc.identifier.issn1476-8305
dc.identifier.urihttps://research.avondale.edu.au/handle/123456789/20598543
dc.language.isoen_us
dc.rights<p>Due to copyright restrictions this article is unavailable for download.</p> <p>The full text of this article may be accessed via ResearchGate <a href="https://www.researchgate.net/publication/280906558_Novel_relationships_between_B12_folate_and_markers_of_inflammation_oxidative_stress_and_NADH_levels_in_the_CNS_and_plasma_of_a_healthy_human_cohort">here</a></p>
dc.subjectCobalamin
dc.subjectFolate
dc.subjectHomocysteine
dc.subjectInflammation
dc.subjectOxidative stress
dc.subjectNicotinamide adenine dinucleotide
dc.subjectCentral nervous system
dc.subjectBrain
dc.titleNovel Relationships between B12, Folate and Markers of Inflammation, Oxidative Stress and NAD(H) Levels, Systemically and in the CNS of a Healthy Human Cohort
dc.typeJournal Article
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