Changes in Oxidative Damage, Inflammation and [NAD(H)] with Age in Cerebrospinal Fluid

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avondale-bepress-to-dspace.facultyNursing
avondale-bepress-to-dspace.licensehttp://creativecommons.org/licenses/by/4.0/
avondale-bepress-to-dspace.peer_review_statusPeer reviewed before publication
avondale-bepress.abstract<p>An extensive body of evidence indicates that oxidative stress and inflammation play a central role in the degenerative changes of systemic tissues in aging. However a comparatively limited amount of data is available to verify whether these processes also contribute to normal aging within the brain. High levels of oxidative damage results in key cellular changes including a reduction in available nicotinamide adenine dinucleotide (NAD+), an essential molecule required for a number of vital cellular processes including DNA repair, immune signaling and epigenetic processing.</p> <p>In this study we quantified changes in [NAD(H)] and markers of inflammation and oxidative damage (F2-isoprostanes, 8-OHdG, total antioxidant capacity) in the cerebrospinal fluid (CSF) of healthy humans across a wide age range (24–91 years). CSF was collected from consenting patients who required a spinal tap for the administration of anesthetic. CSF of participants aged .45 years was found to contain increased levels of lipid peroxidation (F2-isoprostanes) (p = 0.04) and inflammation (IL-6) (p = 0.00) and decreased levels of both total antioxidant capacity (p = 0.00) and NAD(H) (p = 0.05), compared to their younger counterparts.</p> <p>A positive association was also observed between plasma [NAD(H)] and CSF NAD(H) levels (p = 0.03). Furtheranalysis of the data identified a relationship between alcohol intake and CSF [NAD(H)] and markers of inflammation. The CSF of participants who consumed .1 standard drink of alcohol per day contained lower levels of NAD(H) compared to those who consumed no alcohol (p,0.05). An increase in CSF IL-6 was observed in participants who reported drinking .0–1 (p,0.05) and .1 (p,0.05) standard alcoholic drinks per day compared to those who did not drink alcohol. Taken together these data suggest a progressive age associated increase in oxidative damage, inflammation and reduced [NAD(H)] in the brain which may be exacerbated by alcohol intake.</p>
avondale-bepress.articleid1086
avondale-bepress.authorsJade Guest
avondale-bepress.authorsRoss Grant
avondale-bepress.authorsTrevor A Mori
avondale-bepress.authorsKevin D Croft
avondale-bepress.context-key6991708
avondale-bepress.coverpage-urlhttps://research.avondale.edu.au/nh_papers/84
avondale-bepress.document-typearticle
avondale-bepress.field.author_faculty_disciplineNursing
avondale-bepress.field.comments<p><strong></strong> © 2014 Guest et al.</p> <p>At the time of writing <em>Ross Grant</em> was affiliated with Avondale College as a Conjoint Lecturer.</p>
avondale-bepress.field.custom_citation<p>Guest, J., Grant, R., Mori, T. A., & Croft, K. D. (2014). Changes in oxidative damage, inflammation and [NAD(H)] with age in cerebrospinal fluid. <em>PLoS One, 9</em>(1), e85335. doi:10.1371/journal.pone.0085335</p>
avondale-bepress.field.distribution_licensehttp://creativecommons.org/licenses/by/4.0/
avondale-bepress.field.doihttps://doi.org/10.1371/journal.pone.0085335
avondale-bepress.field.embargo_date2015-04-15T00:00:00Z
avondale-bepress.field.field_of_education06 Health
avondale-bepress.field.for119999 Medical and Health Sciences not elsewhere classified
avondale-bepress.field.issn1932-6203
avondale-bepress.field.issue_number1
avondale-bepress.field.journalPLoS One
avondale-bepress.field.page_numberse85335
avondale-bepress.field.peer_reviewBefore publication
avondale-bepress.field.publication_date2014-01-14T00:00:00Z
avondale-bepress.field.reportable_itemsC1
avondale-bepress.field.source_publication<p>This article was originally published as:</p> <p>Guest, J., Grant, R., Mori, T. A., & Croft, K. D. (2014). Changes in oxidative damage, inflammation and [NAD(H)] with age in cerebrospinal fluid. <em>PLoS One, 9</em>(1), 1-8. doi: 10.1371/journal.pone.0085335</p> <p>ISSN: 1932-6203</p>
avondale-bepress.field.staff_classificationPermanent
avondale-bepress.field.volume_number9
avondale-bepress.fulltext-urlhttps://research.avondale.edu.au/cgi/viewcontent.cgi?article=1086&amp;context=nh_papers&amp;unstamped=1
avondale-bepress.keywordsoxidative stress
avondale-bepress.keywordsinflammation
avondale-bepress.keywordsaging
avondale-bepress.keywordsbrain
avondale-bepress.label84
avondale-bepress.publication-date2014-01-14T00:00:00Z
avondale-bepress.publication-titleNursing and Health Papers and Journal Articles
avondale-bepress.statepublished
avondale-bepress.submission-date2015-04-15T17:38:59Z
avondale-bepress.submission-pathnh_papers/84
avondale-bepress.titleChanges in Oxidative Damage, Inflammation and [NAD(H)] with Age in Cerebrospinal Fluid
avondale-bepress.typearticle
dc.contributor.authorCroft, Kevin D.
dc.contributor.authorMori, Trevor A.
dc.contributor.authorGrant, Ross
dc.contributor.authorGuest, Jade
dc.date.accessioned2023-11-01T00:22:35Z
dc.date.available2023-11-01T00:22:35Z
dc.date.issued2014-01-14
dc.date.submitted2015-04-15T17:38:59Z
dc.description.abstract<p>An extensive body of evidence indicates that oxidative stress and inflammation play a central role in the degenerative changes of systemic tissues in aging. However a comparatively limited amount of data is available to verify whether these processes also contribute to normal aging within the brain. High levels of oxidative damage results in key cellular changes including a reduction in available nicotinamide adenine dinucleotide (NAD+), an essential molecule required for a number of vital cellular processes including DNA repair, immune signaling and epigenetic processing.</p> <p>In this study we quantified changes in [NAD(H)] and markers of inflammation and oxidative damage (F2-isoprostanes, 8-OHdG, total antioxidant capacity) in the cerebrospinal fluid (CSF) of healthy humans across a wide age range (24–91 years). CSF was collected from consenting patients who required a spinal tap for the administration of anesthetic. CSF of participants aged .45 years was found to contain increased levels of lipid peroxidation (F2-isoprostanes) (p = 0.04) and inflammation (IL-6) (p = 0.00) and decreased levels of both total antioxidant capacity (p = 0.00) and NAD(H) (p = 0.05), compared to their younger counterparts.</p> <p>A positive association was also observed between plasma [NAD(H)] and CSF NAD(H) levels (p = 0.03). Furtheranalysis of the data identified a relationship between alcohol intake and CSF [NAD(H)] and markers of inflammation. The CSF of participants who consumed .1 standard drink of alcohol per day contained lower levels of NAD(H) compared to those who consumed no alcohol (p,0.05). An increase in CSF IL-6 was observed in participants who reported drinking .0–1 (p,0.05) and .1 (p,0.05) standard alcoholic drinks per day compared to those who did not drink alcohol. Taken together these data suggest a progressive age associated increase in oxidative damage, inflammation and reduced [NAD(H)] in the brain which may be exacerbated by alcohol intake.</p>
dc.description.versionBefore publication
dc.identifier.citation<p>Guest, J., Grant, R., Mori, T. A., & Croft, K. D. (2014). Changes in oxidative damage, inflammation and [NAD(H)] with age in cerebrospinal fluid. <em>PLoS One, 9</em>(1), e85335. doi:10.1371/journal.pone.0085335</p>
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0085335
dc.identifier.issn1932-6203
dc.identifier.urihttps://research.avondale.edu.au/handle/123456789/06991708
dc.language.isoen_us
dc.provenance<p>This article was originally published as:</p> <p>Guest, J., Grant, R., Mori, T. A., & Croft, K. D. (2014). Changes in oxidative damage, inflammation and [NAD(H)] with age in cerebrospinal fluid. <em>PLoS One, 9</em>(1), 1-8. doi: 10.1371/journal.pone.0085335</p> <p>ISSN: 1932-6203</p>
dc.rights<p><strong></strong> © 2014 Guest et al.</p> <p>At the time of writing <em>Ross Grant</em> was affiliated with Avondale College as a Conjoint Lecturer.</p>
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/
dc.subjectoxidative stress
dc.subjectinflammation
dc.subjectaging
dc.subjectbrain
dc.titleChanges in Oxidative Damage, Inflammation and [NAD(H)] with Age in Cerebrospinal Fluid
dc.typeJournal Article
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