Effect of Umbilical Cord Blood Prefreeze Variables on Postthaw Viability

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avondale-bepress-to-dspace.facultyNursing
avondale-bepress-to-dspace.peer_review_statusPeer reviewed before publication
avondale-bepress.abstract<p><h4>Background</h4></p> <p>Assessment of the overall postthaw (PT) viability of an umbilical cord blood (UCB) unit is an important criterion for determining the quality of the unit for transplantation. Overall PT viability is a measure of cellular damage that can occur to the UCB during collection, storage, processing, freezing, and thawing. <h4>Study Design and Methods</h4></p> <p>This study investigated factors measured before freezing of the UCB that could affect overall PT viability of the stem cell unit from 257 collected cord blood samples. The analysis included hematologic variables, cord blood collection characteristics, and stem cell separation and preservation factors. <h4>Results</h4></p> <p>Each of the variables, postprocess (PP) neutrophils (%), PP hematocrit, overall PP viability (%), freeze rate (°C/min), and time from collection to freezing (hr) were shown to contribute to overall PT viability. Each UCB sample was given a calculated “viability prediction” (VP) score based on the influence or impact of each of these variables. This score was compared to the measured PT viability. Variables with a low VP score had correspondingly low PT viability, indicating more overall damage to the cells. The results showed that the higher the VP score, the higher the PT viability. <h4>Conclusion</h4></p> <p>These findings provide a framework for identifying those units that are most likely to have a high overall PT viability and hence an increased likelihood of successful engraftment of the CB-sourced stem cells. The VP score could aid in the selection of a donor cord blood unit for transplantation.</p>
avondale-bepress.articleid1091
avondale-bepress.authorsBelinda Pope
avondale-bepress.authorsBevan Hokin
avondale-bepress.authorsRoss Grant
avondale-bepress.context-key7102202
avondale-bepress.coverpage-urlhttps://research.avondale.edu.au/nh_papers/89
avondale-bepress.document-typearticle
avondale-bepress.field.author_faculty_disciplineNursing
avondale-bepress.field.avon_earlyonline2014-10-21T00:00:00-07:00
avondale-bepress.field.comments<p>Due to copyright restrictions this article is unavailable for download.</p> <p>© 2014 AABB</p> <p>This article may be accessed from the publisher <a href="http://dx.doi.org/10.1111/trf.12873">here.</a></p> <p>At the time of writing <em>Ross Grant</em> was affiliated with Avondale College of Higher Education as a Conjoint Lecturer.</p>
avondale-bepress.field.custom_citation<p>Pope, B., Hokin, B., & Grant, R. (2015). Effect of umbilical cord blood prefreeze variables on postthaw viability. <em>Transfusion, 55</em>(3), 629–635. doi:10.1111/trf.12873</p>
avondale-bepress.field.doihttps://doi.org/10.1111/trf.12873
avondale-bepress.field.email_boxtrue
avondale-bepress.field.embargo_date2015-05-13T00:00:00Z
avondale-bepress.field.field_of_education06 Health
avondale-bepress.field.for111499 Paediatrics and Reproductive Medicine not elsewhere classified
avondale-bepress.field.issn1537-2995
avondale-bepress.field.issue_number3
avondale-bepress.field.journalTransfusion
avondale-bepress.field.page_numbers629-635
avondale-bepress.field.peer_reviewBefore publication
avondale-bepress.field.publication_date2015-03-11T00:00:00Z
avondale-bepress.field.source_fulltext_urlhttps://doi.org/10.1111/trf.12873
avondale-bepress.field.source_publication<p>This article was originally published as:</p> <p>Pope, B., Hokin, B., & Grant, R. (2015). Effect of umbilical cord blood prefreeze variables on postthaw viability. <em>Transfusion, 55</em>(3), 629–635. doi: 10.1111/trf.12873</p> <p>ISSN:1537-2995</p>
avondale-bepress.field.staff_classificationPermanent
avondale-bepress.field.volume_number55
avondale-bepress.label89
avondale-bepress.publication-date2015-03-11T00:00:00Z
avondale-bepress.publication-titleNursing and Health Papers and Journal Articles
avondale-bepress.statepublished
avondale-bepress.submission-date2015-05-13T19:44:15Z
avondale-bepress.submission-pathnh_papers/89
avondale-bepress.titleEffect of Umbilical Cord Blood Prefreeze Variables on Postthaw Viability
avondale-bepress.typearticle
dc.contributor.authorGrant, Ross
dc.contributor.authorHokin, Bevan
dc.contributor.authorPope, Belinda
dc.date.accessioned2023-11-01T00:22:38Z
dc.date.available2023-11-01T00:22:38Z
dc.date.issued2015-03-11
dc.date.submitted2015-05-13T19:44:15Z
dc.description.abstract<p><h4>Background</h4></p> <p>Assessment of the overall postthaw (PT) viability of an umbilical cord blood (UCB) unit is an important criterion for determining the quality of the unit for transplantation. Overall PT viability is a measure of cellular damage that can occur to the UCB during collection, storage, processing, freezing, and thawing. <h4>Study Design and Methods</h4></p> <p>This study investigated factors measured before freezing of the UCB that could affect overall PT viability of the stem cell unit from 257 collected cord blood samples. The analysis included hematologic variables, cord blood collection characteristics, and stem cell separation and preservation factors. <h4>Results</h4></p> <p>Each of the variables, postprocess (PP) neutrophils (%), PP hematocrit, overall PP viability (%), freeze rate (°C/min), and time from collection to freezing (hr) were shown to contribute to overall PT viability. Each UCB sample was given a calculated “viability prediction” (VP) score based on the influence or impact of each of these variables. This score was compared to the measured PT viability. Variables with a low VP score had correspondingly low PT viability, indicating more overall damage to the cells. The results showed that the higher the VP score, the higher the PT viability. <h4>Conclusion</h4></p> <p>These findings provide a framework for identifying those units that are most likely to have a high overall PT viability and hence an increased likelihood of successful engraftment of the CB-sourced stem cells. The VP score could aid in the selection of a donor cord blood unit for transplantation.</p>
dc.description.versionBefore publication
dc.identifier.citation<p>Pope, B., Hokin, B., & Grant, R. (2015). Effect of umbilical cord blood prefreeze variables on postthaw viability. <em>Transfusion, 55</em>(3), 629–635. doi:10.1111/trf.12873</p>
dc.identifier.doihttps://doi.org/10.1111/trf.12873
dc.identifier.issn1537-2995
dc.identifier.urihttps://research.avondale.edu.au/handle/123456789/07102202
dc.language.isoen_us
dc.provenance<p>This article was originally published as:</p> <p>Pope, B., Hokin, B., & Grant, R. (2015). Effect of umbilical cord blood prefreeze variables on postthaw viability. <em>Transfusion, 55</em>(3), 629–635. doi: 10.1111/trf.12873</p> <p>ISSN:1537-2995</p>
dc.rights<p>Due to copyright restrictions this article is unavailable for download.</p> <p>© 2014 AABB</p> <p>This article may be accessed from the publisher <a href="http://dx.doi.org/10.1111/trf.12873">here.</a></p> <p>At the time of writing <em>Ross Grant</em> was affiliated with Avondale College of Higher Education as a Conjoint Lecturer.</p>
dc.titleEffect of Umbilical Cord Blood Prefreeze Variables on Postthaw Viability
dc.typeJournal Article
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