Long Term Persistence of Nitrosamine-Induced Structural Damage to Heterochromatic DNA

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avondale-bepress-to-dspace.facultyScience
avondale-bepress.abstract<p>Different levels of damage and repair to eu- and heterochromatic DNA from the livers of rats receiving a dose of 10 mg/kg N-nitrosodimethylamine (NDMA) were apparent. Preincorporated <sup>3</sup>H~thymidine was lost rapidly from euchromatic DNA but persisted in the heterochromatic fraction. Persistent damage, determined as single-stranded regions binding 10 benzoylated DEAE-cellulose (BD-cellulose), was evident in heterochromatic DNA for up to three months. By subjecting rats treated with NDMA to partial hepatectomy, generation of single-stranded regions in the newly synthesized heterochromatic DNA could be demonstrated. Such structural defects were evident when hepatectomy was performed two months after administration of the carcinogen. These findings indicate that structural damage to non-transcribed DNA is one of the most persistent molecular lesions following exposure to nitrosamines.</p>
avondale-bepress.articleid1001
avondale-bepress.authorsBernard W Stewart
avondale-bepress.authorsEwan Ward
avondale-bepress.context-key6379809
avondale-bepress.coverpage-urlhttps://research.avondale.edu.au/sci_math_conferences/2
avondale-bepress.document-typeconference
avondale-bepress.field.author_faculty_disciplineScience
avondale-bepress.field.comments<p>Due to copyright restrictions this conference proceeding is unavailable for download.</p> <p>This conference proceeding may be accessed from the publisher <a href="https://www.iarc.fr/">here.</a></p> <p>This book can be accessed from the National Library of Australia <a href="http://catalogue.nla.gov.au/Record/233357">here</a></p>
avondale-bepress.field.custom_citation<p>Stewart, B. W., & Ward, E. J. (1987). Long term persistence of nitrosamine-induced structural damage to heterochromatic DNA. In H. Bartsch, I. K. O'Neil, & R. Schulte-Hermann (Eds.), <em>Relevance of N-nitroso compounds to human cancer: Exposures and mechanisms. </em>Paper presented at the International Symposium on N-nitroso Compounds, Baden, 1-5 September (pp. 64-67). Lyon, France: International Agency for Research on Cancer.</p>
avondale-bepress.field.email_boxtrue
avondale-bepress.field.embargo_date2014-11-20T00:00:00Z
avondale-bepress.field.field_of_education01 Natural and Physical Sciences
avondale-bepress.field.for060199 Biochemistry and Cell Biology not elsewhere classified
avondale-bepress.field.proceedingsInternational Symposium on N-nitroso Compounds
avondale-bepress.field.publication_date1987-01-01T00:00:00Z
avondale-bepress.field.source_publication<p>This conference presentation was originally published as:</p> <p>Stewart, B. W., & Ward, E. J. (1987). Long term persistence of nitrosamine-induced structural damage to heterochromatic DNA. In H. Bartsch, I. K. O'Neil, & R. Schulte-Hermann (Eds.), <em>Relevance of N-nitroso compounds to human cancer: Exposures and mechanisms. </em>Paper presented at the International Symposium on N-nitroso Compounds, Baden, 1-5 September (pp. 64-67). Lyon, France: International Agency for Research on Cancer.</p> <p>ISBN: 9789283211846</p>
avondale-bepress.field.staff_classificationPermanent
avondale-bepress.keywordsbiology
avondale-bepress.keywordscancer
avondale-bepress.label2
avondale-bepress.publication-date1987-01-01T00:00:00Z
avondale-bepress.publication-titleScience and Mathematics Conference Papers
avondale-bepress.statepublished
avondale-bepress.submission-date2014-11-20T17:34:57Z
avondale-bepress.submission-pathsci_math_conferences/2
avondale-bepress.titleLong Term Persistence of Nitrosamine-Induced Structural Damage to Heterochromatic DNA
avondale-bepress.typearticle
dc.contributor.authorWard, Ewan
dc.contributor.authorStewart, Bernard W.
dc.date.accessioned2023-11-01T00:38:46Z
dc.date.available2023-11-01T00:38:46Z
dc.date.issued1987-01-01
dc.date.submitted2014-11-20T17:34:57Z
dc.description.abstract<p>Different levels of damage and repair to eu- and heterochromatic DNA from the livers of rats receiving a dose of 10 mg/kg N-nitrosodimethylamine (NDMA) were apparent. Preincorporated <sup>3</sup>H~thymidine was lost rapidly from euchromatic DNA but persisted in the heterochromatic fraction. Persistent damage, determined as single-stranded regions binding 10 benzoylated DEAE-cellulose (BD-cellulose), was evident in heterochromatic DNA for up to three months. By subjecting rats treated with NDMA to partial hepatectomy, generation of single-stranded regions in the newly synthesized heterochromatic DNA could be demonstrated. Such structural defects were evident when hepatectomy was performed two months after administration of the carcinogen. These findings indicate that structural damage to non-transcribed DNA is one of the most persistent molecular lesions following exposure to nitrosamines.</p>
dc.identifier.citation<p>Stewart, B. W., & Ward, E. J. (1987). Long term persistence of nitrosamine-induced structural damage to heterochromatic DNA. In H. Bartsch, I. K. O'Neil, & R. Schulte-Hermann (Eds.), <em>Relevance of N-nitroso compounds to human cancer: Exposures and mechanisms. </em>Paper presented at the International Symposium on N-nitroso Compounds, Baden, 1-5 September (pp. 64-67). Lyon, France: International Agency for Research on Cancer.</p>
dc.identifier.urihttps://research.avondale.edu.au/handle/123456789/06379809
dc.language.isoen_us
dc.provenance<p>This conference presentation was originally published as:</p> <p>Stewart, B. W., & Ward, E. J. (1987). Long term persistence of nitrosamine-induced structural damage to heterochromatic DNA. In H. Bartsch, I. K. O'Neil, & R. Schulte-Hermann (Eds.), <em>Relevance of N-nitroso compounds to human cancer: Exposures and mechanisms. </em>Paper presented at the International Symposium on N-nitroso Compounds, Baden, 1-5 September (pp. 64-67). Lyon, France: International Agency for Research on Cancer.</p> <p>ISBN: 9789283211846</p>
dc.rights<p>Due to copyright restrictions this conference proceeding is unavailable for download.</p> <p>This conference proceeding may be accessed from the publisher <a href="https://www.iarc.fr/">here.</a></p> <p>This book can be accessed from the National Library of Australia <a href="http://catalogue.nla.gov.au/Record/233357">here</a></p>
dc.subjectbiology
dc.subjectcancer
dc.titleLong Term Persistence of Nitrosamine-Induced Structural Damage to Heterochromatic DNA
dc.typeConference Publication
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